The shelf-life quality of the leaves was evaluated according to
changes in freshness and yellowing of leaves. Leaves were assessed
daily for visual yellowing and freshness. The visual yellowing was
scored according to the following scale: 0–25%, 25–50%, 50–75%
and 75–100% of yellowing. When the leaves showedmore than 50%
yellowing, theywere considered to have reached to the end of their
shelf-life.