The time has entered the 21st century when the biological and
material science has been flourishing as never before. Many hot
research fields call for the sensitive analysis of target compounds,
especially organic compounds, from complicated samples. A lot of
emphasis has been put on the development of separation techniques,
particularly liquid chromatography (LC). Nowadays, LC has
been considered as a powerful tool for the separation and analysis
of organic components from complicated samples. The target
compounds for LC separation are almost non- or weak-volatile
compounds that cover almost 75% of target compounds in routine
and scientific analyses.