Fig. 2. Effect of incubation temperature on C. perfringens spore germination. Heatactivated
spores of NCTC8239 were incubated with BHI broth plus 50 mM AK at 37 C
(open bar) or 55 C (filled bar), and at various times aliquots plated onto BHI agar,
survivals determined and DR value calculated as described in Materials and methods.