Background: Vegetative propagation of Fragaria sp. is traditionally carried out using stolons. This system of propaga‑tion, in addition to being slow, can spread plant diseases, particularly serious being viral. In vitro culture of meristemsand the establishment of micropropagation protocols are important tools for solving these problems. In recent years,considerable efort has been made to develop in vitro propagation of the commercial strawberry in order to producevirus-free plants of high quality. These previous results can serve as the basis for developing in vitro-based propaga‑tion technologies in the less studied species Fragaria chiloensis.