Effect of Temperature on the Separation of Anthocyanins.
Following the choice of column and mobile phase, the efficiency
of separation can be enhanced by increasing the separation
temperature. In our previous study, anthocyanins in blue wheat
either in whole meal or in isolated form were thermally stable
at pH 1 and 65 °C, but as the temperature increased to 95 °C
the degradation of anthocyanins increased (3). To prevent any
thermal degradation during the analysis of anthocyanins and to
maintain column quality, the highest temperature employed was
50 °C. Three column temperatures (25, 38, and 50 °C)