Strains containing individual folate biosynthesis genes under the control of the
PGK1 promoter, and the wild-type M2 strain, were fermented for two days in white
wine (Chardonnay) and red wine (Pinot Noir) grape must. Yeast cells were collected,
and total RNA isolated. The expression levels of each altered gene were
measured by qPCR, as described in Section 2. Numbers indicate fold overexpression
compared to wild-type. Numbers in parentheses indicate the bounds of the 95%
confidence interval