The glutamic and aspartic acids are present at 18.99 g/100 g and 11.95 g/100 g, respectively, in red tomato seed meal . They are known as umami taste amino acids or monosodium glutamate like (MSG-like) amino acids . These facts imply that the tomato seed meal could be used to develop products rich in umami flavors for both vegetarians and non-vegetarians. However, little research has been done on the development and optimization of processes for preparing flavor components from DTSM using papain hydrolysis and this research will be the first of its kind. Previous investigations revealed that use of papain resulted in a higher degree of hydrolysis of whey and egg protein compared with the other enzymes such as pepsin and trypsinase . Therefore, papain was used for hydrolysis of protein from DTSM in this study.