General procedure for phytosterol content in hydroethanolic
extract
Hydroethanolic extract (100 ml) was dried under reduced
pressure at 40°C. The residue was resuspended in 20 ml of
chloroform and the volume adjusted to 50 ml with the same
solvent (SM). SM aliquots were transferred to 10 ml volumetric
flasks and 2 ml of LB reagent was added. The volume was
adjusted with chloroform. The absorptions were measured in a
spectrophotometer UV/Vis (Evolution 60S, Thermo Scientific®,
Germany), 5 min after the addition of the reagent LB. The
chloroform was used as a blank.