In addition, 40-mL aliquots of the inoculated milk were aseptically distributed
in 50-mL sterile flasks. One flask was prepared for each sampling
time and it was used for all analyses. The pH value, organic acids
and lactose contents were determined in the incubated milk before fermentation,
at pH values of 6.0, 5.5, 5.0 and 4.6 (end of fermentation).