Split soybeans (150 g) and 73.5 ml of distilled water were separately
placed in 500 ml capped Duran bottles and autoclaved at
121 C for 20 min. After the soybeans and the distilled water cooled
down (at room temperature) they were both mixed with the spore
suspension and manually shaken (vertically and horizontally) for
10 min to homogenise the inoculum. The inoculated soybeans
were poured into Petri dishes and incubated at 30 C for 5 days.
Soybean samples were crushed with a mortar and pestle before
being sealed in plastic bags and store at 30 C until used.