In 1994, a GFP-based reporter system was developed in which visible fluorescence was created by molecular biological techniques. Prashar et al. (1992) cloned gdf cDNA from Aequorea victoria and Chalfie et al. (1994) showed the expression of the cloned GFP gene produces green fluorescence in various organisms such as Drosophila and Escherichia coli. The GPF can be used as a reporter for the visualization of gene expression and protein subcellular localization (Chalfie et al., 1994). Inouye and Tsuji (1994b) showed that the GPF can be introduced into cells and intact organelles within cells.