The separation of photosynthetic pigments was done
according to reference
[18]
. Brie
fl
y, the extract was prepared
by homogenization of 2 g of the spinach leaves in a crucible
mortar with the aid of quartz sand and sodium carbonate
(0.5 g). Homogenization was done twice with the addition
of 5 ml of acetone each time. The macerate was
fi
ltered
through an Advantec Toyo (No. 2)
fi
lter paper, and further
debris was removed by centrifugation with a Beckman J2-
MC Centrifuge (USA) at 12,100
g
for 20 min. The
quanti
fi
cation of Chl
a
, Chl
b
, and total Cars in the acetone
supernatants as well as separation of photosynthetic
pigments was done according to the reference
[18]
.
Furthermore, according to reference
[5]
, a mobile phase
consisting of acetonitrile:methanol:water:ethyl acetate
(7.00:0.96:0.04:2.00, v/v) was used for separation. The
injection volume was 10
m
l with the solvent
fl
ow rate of
16.6
m
ls
1
for 15 min. Peak identi
fi
cation for various
photosynthetic pigments was done by injecting known
amounts of pure pigments, and plotting peak area versus
mass of the pigment injected as described in reference
[5]
.
The separation of photosynthetic pigments was done
according to reference
[18]
. Brie
fl
y, the extract was prepared
by homogenization of 2 g of the spinach leaves in a crucible
mortar with the aid of quartz sand and sodium carbonate
(0.5 g). Homogenization was done twice with the addition
of 5 ml of acetone each time. The macerate was
fi
ltered
through an Advantec Toyo (No. 2)
fi
lter paper, and further
debris was removed by centrifugation with a Beckman J2-
MC Centrifuge (USA) at 12,100
g
for 20 min. The
quanti
fi
cation of Chl
a
, Chl
b
, and total Cars in the acetone
supernatants as well as separation of photosynthetic
pigments was done according to the reference
[18]
.
Furthermore, according to reference
[5]
, a mobile phase
consisting of acetonitrile:methanol:water:ethyl acetate
(7.00:0.96:0.04:2.00, v/v) was used for separation. The
injection volume was 10
m
l with the solvent
fl
ow rate of
16.6
m
ls
1
for 15 min. Peak identi
fi
cation for various
photosynthetic pigments was done by injecting known
amounts of pure pigments, and plotting peak area versus
mass of the pigment injected as described in reference
[5]
.
การแปล กรุณารอสักครู่..