The Hop stunt viroid (HSVd), a member of the Hostuviroid genus within the Pospiviroidae family, is a typical viroid species that infects the common hop plants, citrus plants, and grapevines. The genome of HSVd contains five structural domains: two terminal regions, left (TL) and right (TR), pathogenic (P), variable (V), and central (C) domains, with a central conserved region (CCR)