Fig. 6. HCdc14A localization in various stages of cell cycle using confocal microscopy. (A) HEK239T cells were transfected with GFP alone or GFP-hCdc14A for 72 h and captured in interphase. Cells were labeled for the GFP fluorescence (green) and the cell membrane was stained using PKH26 (red). (B) HeLa cells were transfected with GFP-hCdc14A for 72 h and captured in various stages of mitosis. Cells were stained for GFP-hCdc14A (green), F-actin (red), and nuclei (DAPI; blue). Representative images of cells in various stages of cell cycle using confocal microscopy were shown. HCdc14A was localized mainly to interphase centrosomes (A), and mitotic spindles and centrosomes (B).