with slight modifications. Two gram of
green chilli paste was taken in 100 ml ethyl acetate and
allowed it to stand for 24 h, from that an extract of 1 ml was
taken and made the volume to 5 ml using ethyl acetate, to that
extract, 0.5 ml of vanadium oxy chloride (Sigma Aldrich, India)
solution (vanadium oxychloride 0.5% in ethyl acetate) was
added just before taking the absorbance of the sample. Pure
capsaicin (0.01%) in ethyl acetate (10 mg in 100 ml) was taken
as standard. The absorbance of the blank, standard and
sample was read at 720 nm. A colour change was observed
after the addition of vanadium oxychloride, the extract
changed from green to yellow colour. The readings were
taken when the extract was in green colour.