The level of reducing sugar in GRR was significantly higher, than
that in UGR and GBR, by approximately 17.25 times (863%) and
1.58 times (79.2%), respectively, whilst that of GBR was 8.74 times
(437%) higher, than that of UGR. The same trends were found in the
level of total sugars (Fig. 1). The increase of reducing sugar and total
sugars is due to the starch degradation presumably being involved
in the initial action of a-amylase on the starch granules,
since a-amylase showed a greater increase in activity (discussed
in next section). The other hydrolyses probably assisted in the
complete hydrolysis to simple sugar and reducing sugar, such as
the action of invertase, which hydrolysis sucrose, to glucose and
fructose. According to Traore et al. (2004), glucose and fructose
were the major reducing sugars in germinated sorghum, millet
and corn, where the level of glucose and fructose of sorghum
was increased by 89.6 folds (4478%) and 30.34-folds (1517%),
respectively.