The mud crabs were dividedin to five groups.The first group acted as control where by this group did not receive any hormone injections.The nextgroup (T1D1)is the treatment of 17α-hydroxy progesterone with concentrations of 0.01 μg/g BW. The next treatment (T1D2) is the treatment of 17α-hydroxy progesterone with concentrations of 0.1 μg/g BW. The next group (T2D1) is the treatment of 17α-hydroxy pregnenolone with the concentrations of0.01μg/gand the last treatment (T2D2) is the treatment of 17α-hydroxy pregnenolone with concentrations of 0.01 μg/gBW.