The aim of this the work was evaluated effect of disinfection of Oravka hatching eggs by formalin gas (20 g KMnO4
+ 30 g formaldehyde of 40 % concentration to 1 m3 of a space) and ozone (0.450 ppm in time 12 hours) on fertility,
hatchability, spontaneity of chickens hatching and embryonal mortality during incubation. The hatching was realised
in Bios Midi incubator at a standard technology process of the hatching. We recorded a positive effect of disinfection
of ozone on hatchability from fertilised eggs (91.79%) compared with disinfection of formalin (91.10%) A totally
embryonal mortality of chickens had not record any difference between two methods of disinfection but we recorded
positive effect of ozone on embryonic mortality of chicks to 7 day of incubation and from 15 to 21 day of incubation.
We searched more spontaneous chicks hatching from hatching eggs disinfected by ozone in 510 hours of incubation.
It is possible to recommend disinfection of hatching eggs by ozone, as an alternative way of disinfection of hatching
eggs usable in hatching practice, on the base of obtained experimental results. This way of disinfection hatching eggs
is more ecological not only from the point of environment, but also of the healthy of employees of the hatchery.