Map of pBS–pds S–ble KpnI, XhoI at the 5′ end/BamHI, XbaI at the 3′ end. The ble CDS of pGenD–ble is designated in yellow; the C. reinhardtii PsaD promoter and 3′ UTR are designated in pink. Restriction sites are indicated. Note that the ble cassette was inserted either at the 5′ of pds (KpnI, XhoI) or at the 3′end (BamHI, XbaI). PCR primers are indicated by F and R.