An alternative perspective for analyzing the ability of amplicons
to distinguish among species uses phylogenetic trees.
Figures S1 and S2 in the supplemental material show phylogenetic
trees for amplicons 28S(10F-12R) and ITS2(5.8SF-
1R), respectively, based on 51 fungi. Note that the ITS2(5.8SF-
1R) amplicon is highly polymorphic and lacks the property of
a molecular clock, making it unreliable for inferring evolutionary
relationships. The trees demonstrate that closely related
fungi are resolved using the proposed amplicon sequences.