Vaccinationwas carried out by intracoelomic injection
of 0.1 ml of non-adjuvanted (NA) aqueous-based suspension containing
formalin-inactivated A. salmonicida strain 1.50 (NA-vaccinated
group) and the oil-adjuvanted (OA) vaccine (OA-vaccinated group),
while the control groups received tryptone soy broth with 0.3% formaldehyde
(TSB-3) emulsified with the non-mineral oil adjuvant (OA-control
group) or TSB-3 alone (NA-control group) (Table 1). All the
injection procedures were performed with the needle directed towards
the fronto-ventral side of the peritoneal cavity (“forward direction”)
(Noia et al., 2014).