The higher dilutions containing Halobacteriovorax
were Vibrio free and were used in enrichments and plaque assays with
other Vibrio and non-Vibrio bacteria to determine the host specificity of
all four Halobacteriovorax strains that were originally isolated in V. parahaemolyticus.
This method was also employed to separate Halobacteriovorax
from E. coli and Salmonella host cells where the absence of host cells
in the filtered diluent was confirmed by spread plating 100-l portions
onto LB agar followed by incubation at 37°C.