Bioassays performed against second-instar whiteflies revealedthat ESALQ1432 blastospores produced in media with higherglucose concentrations (140 g L−1) resulted in higher virulencewhen compared with blastospores produced in media with 40 gglucose L−1 (Table 4). The ratio test comparing LC50 valuesrevealed that blastospores harvested from media supplementedwith 140 g glucose L−1 exhibited twofold-improved insecticidalactivity compared with blastospores produced in media with40 g glucose L−1. Virulence (LC50) of blastospores producedin media containing 100 g glucose L−1 was intermediate. Consistentwith LC50 results, the concentration-mortality responsecurve for blastospores was significantly different when producedin media with 140 g glucose L−1 as compared with40 g glucose L−1 (F(3, 77)=3.09, P=0.032) suggesting a higherinsecticidal performance for blastospores produced in mediumcontaining higher concentrations of glucose (Fig. 6). The proportionof whiteflies surviving all blastospore treatments wassignificantly lower when compared with the control group(χ2(3)=990.59, P<0.0001).Moreover, the survival analysis suggesteda faster insecticidal activity for blastospores produced inmedium with 140 g glucose L−1 in relation to blastospores
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