2.7. Bread firmness
Firmness of bread was determined using AACC method No.74-
09 standard method (AACC, 1995). using The Stable Micro Systems
texture analyser TA-XT2i equipped with 50 kg load cell in
compression mode with a 36 mm diameter cylindrical probe. The
texture analyser was set at a pre and post test speed of 1 mm/s and
1.7 mm/s respectively.
2.8. Bread quality characteristics
Loaf weight and volume of bread samples were measured on 1 h
after removal from the oven. Loaf volume was determined by the
rapeseed displacement method. Specific volume was calculated by
dividing loaf volume by loaf weight (Koca & Anil, 2007).
2.9. Statistical analysis
The data were subjected to analysis of variance (ANOVA test)
using SPSS for windows. Means were separated by Fisher’s protected
least significant difference (LSD). The significant level was
established at p 0.05.
3. Results and discussion
3.1. Proximate analysis of raw materials
The proximate analysis of raw material in present study is given
in the Table 1. From table it can be seen that flaxseed flour showed
highest fat content (40.5) compared to whole wheat flour (2.36)
and refined wheat flour (2.33). The protein content in refined
wheat flour was about 10.37(g/100 g) where as in whole wheat