Antibiotic is one of the most important commercially exploited secondary metabolites produced by
bacteria and employed in a wide range. Most of the antibiotics used today are from the microbes. Bacteria are
easy to isolate, culture, maintain and to improve their strain. Bacillus species being the predominant soil bacteria
because of their resistant endospore formation and production of vital antibiotic like bacitracin etc. are always
found inhibiting the growth of the other organisms. In the present research study, soil bacteria with the
antibiotic activity was screened and isolated. The media used in this research was nutrient agar medium. 1g of
the soil samples were dissolved in 10ml of sterile water to make soil suspensions. Portion of the suspensions
were inoculated on the nutrient agar by streaking and were incubated at 37°C for 24 hours. After which colonies
with a clear zone of inhibition were observed. The bacteria isolated were; Bacillus lentus, Micrococcus roseus,
Bacillus alvei, Enterobcteraerogene and Bacillus pumillus. The inhibitory activities of the isolated
microorganisms were checked against some of the important opportunistic microflora like Staphylococcus
aureus and Pseudomonas species.