1116 and Salmonella typhi (S. typhi) ATCC 700931 were
used as test organisms. The test organisms were inoculated
perpendicular to the antagonist on the agar medium. The
plates were incubated at 37 曟 for 24 h. The microbial
inhibitions were observed by determining the diameter
of the inhibition zones[10]. Of all the isolates, two best
antagonistic Actinomycetes isolates were selected, identified
macroscopically and microscopically by Gram’s staining and
used for further studies.