In the second phase of the experiment, 50 dah-stage fish that
were past gonadal sexual differentiation were used. All individuals
before the start of the experiment had immature ovaries with an
ovarian cavity and few oocytes at the perinucleolar stage
(Fig. S2A). Forty-five days after treatment, control fish had ovaries
with the perinucleolar and previtellogenic-stage oocytes (Fig. S2B),
while the ovaries of all fish in the 37C treated group had no germ
cells (Fig. S2C). Following transfer of fish to normal temperature
and additional five months of rearing, all fish in the control group
had normal ovaries with mature stage oocytes (Fig. S2D, S2E). In
contrast, all fish in the 37C group had thread-like paired ovaries
without germ cells (Fig. S2F, S2G) and supporting somatic cells
and ovarian cavity were not affected.
In the second phase of the experiment, 50 dah-stage fish thatwere past gonadal sexual differentiation were used. All individualsbefore the start of the experiment had immature ovaries with anovarian cavity and few oocytes at the perinucleolar stage(Fig. S2A). Forty-five days after treatment, control fish had ovarieswith the perinucleolar and previtellogenic-stage oocytes (Fig. S2B),while the ovaries of all fish in the 37C treated group had no germcells (Fig. S2C). Following transfer of fish to normal temperatureand additional five months of rearing, all fish in the control grouphad normal ovaries with mature stage oocytes (Fig. S2D, S2E). Incontrast, all fish in the 37C group had thread-like paired ovarieswithout germ cells (Fig. S2F, S2G) and supporting somatic cellsand ovarian cavity were not affected.
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