The system repeatability of the method was estimated by 36
determinations with 6 replicate injections of a standard solution.
Six different concentrations of a standard solution were tested
(0.010, 0.025, 0.050, 0.100, 0.200 and 0.300 lg/ml). The relative
standard deviation (% RSD) of the analytes ranges from 7.37% (for
the lowest standard elution concentration) to 0.53% (for the highest
standard elution concentration) .
The assay repeatability was estimated with 6 replicate samples
from homogenous powder mixtures of Hippophae rhamnoides
plants which were analyzed according to the described assay.
The relative standard deviation (% RSD) of the analyte was 0.7%.
The intermediate repeatability was determined by three independent
analysts, by analyzing the same powder mixtures of
Hippophae rhamnoides, and the % RSD was low and the method
precision therefore acceptable