The assay mixture contained 250 L p-nitrophenol (0.3 mM) in 10 mM KMES pH 6.2, 250 L substrates (glucose and fructose, each 0.5 M in the same buffer) and 50 L gluconolactonase (90–120 U mL−1)
The assay mixture contained 250 L p-nitrophenol (0.3 mM) in 10 mM KMESpH 6.2, 250 L substrates (glucose and fructose, each 0.5 M in the same buffer)and 50 L gluconolactonase (90–120 U mL−1)