The effect of different soiless substrates (viz. agrotextile, coir fibre, perlite and rock wool) on strawberryproductivity and fruit composition of three strawberry cultivars (‘Camarosa’, ‘Candonga’ and ‘Festival’)was investigated.Plant productivity and berry composition was mainly affected by differences between cultivars ratherthan by the nature of the different growing substrates. However, regardless of the cultivar, plants grownon agrotextile-type substrate produced significantly more fruit (1018.2 g plant−1) than those grown inother substrates (average 892.3 g plant−1). In addition, accompanying greater fruit production, fruit fromplants grown on agrotextile generally had the lowest concentrations of the main strawberry anthocyaninsperlargonidin-3-glucoside (0.74-fold) and pelargonidin derivative 1 (0.85-fold). Nor sugars, organic acidsor any other health-related compounds were significantly affected by the nature of the soilless substrate.Overall, the present study demonstrates that despite some minor differences in fruit yield and thehealth-related composition of fruit grown on agrotextile, a number of different substrates with differentphysico-chemical characteristics may be employed during soilless cultivation of strawberry fruit withoutdetrimentally affecting final fruit quality