Each reaction contains 9 L of SYBR-Green supermix, 2 L of cDNA template, 1 L of 6 M forward and reverse primers, and 7 L of sterile water. The qPCR program consists of 1 cycle at 95◦C, followed by 40 cycles of15 s at 95◦C, 45 s at 56◦C, and 50 s at 68◦C.