Introduction
Environmental contamination with pesticides is
a problem of world-wide importance. Data on
their accumulation by fish are therefore valuable
to assess both the safety of pesticides for man
and the extent of contamination of the fish in
the environment. In general, evaluation of pesticide
accumulation in fish has been carried out
for individual pesticides but not for complex
mixtures, as is evident from the OECD Guidelines
of Testing of Chemicals (1981) and “The
law concerning the examination and regulation
of manufacture etcetera test of chemical substances”
in Japan. The same applies to their
toxicity.
In the case of pesticide toxicity to fish, the
influence of single and complex mixtures used to
treat golf courses has been tested on killifish,
and the enhancement of the complex toxicity
was recognized in several combinations of
two or three pesticides such as (diazinon +
captan) and (diazinon + fenitrothion +