Studies imply that 15–30% increase in lipid content would decrease the growth rate up to 50%. Rodolfi et al. viewed that 22% of the nutrients is used for biomass production in the plants and the rest is used oil production [93]. Lipids are divided into three categories: neutral lipids, crude lipids, and total lipids. Neutral lipids contain triglycerides, free fatty acids, hydrocarbons, alcohols, esters and wax. Crude lipids and total lipids contain pigments. Total lipids also possess phospholipids and glycolipids [4].