A two-dimensional titer determination for the optimum dilution of GH label and the antiserum was carried
out. Antibody dilutions ranging from 1:5000 to 1:640,000 and the biotinyl-GH dilutions of 1:500 to 1:16,000 were
tested. The antibody titer of 1:40,000 and the biotinyl-GH conjugate titer of 1:3000 were found to be the most
suitable and achieved an OD450 of around 1.1. rates (r=0.73 between growth rate and plasma GH
levels). As the age and BW increased, the growth rates decreased gradually up to G-III, remained static
thereafter up to G-VIII, and again decreased from there and found to be similar in rest of the groups
(pN0.05).