The advantages of the proposed bioprocess in this study clearly showed that 85% of carbohydrate recovery from mixed food and bakery waste through fungal hydrolysis was achieved. Furthermore, L. casei Shirota can produce up to 94.0 g L−1 LA with a yield of 0.94 g g−1 and a productivity of 2.61 g L−1 h−1, and no acid by-products were found in the L. casei Shirota fermentation