This work reports on supercritical CO2 extraction of lycopene from tomato peel by-product carried out with the use of indigenous oil from tomato seed obtained along with the tomato peel. The objectives of our study were to determine the effect of temperature, pressure, and CO2 flow rate on lycopene recovery from tomato peel in the presence of tomato seed oil as a co-solvent. We considered that the presence of tomato seed oil can improve the extraction of lycopene and have a beneficial role in the stability of lycopene due to its fatty acid content.