each solution was placed in 10 ml test tubes. Leaf tissue from the mid-top leaves of the plant were cut using a scalpel into 10 small pieces (each piece: 0.2–0.5 cm2) and placed into 10 ml test tubes with 2.0 ml of each solution. Tissue was incubated in solution for approximately 30 min. Using a dissecting needle to dip a bit methylene blue dye (approximately 0.001 g) and added it into every 10 ml test tube. Using a glass pipette a drop of the solution bathing the leaf tissue was withdrawn and released in 20 ml test tubes.