A batch operation for 10 days had been conducted concurrently to establish biofilms
and clogging test. For the batch operation, six liter of mixed sample was applied into the model The influent effluent ration must 1:1 (clogging index ≈1) to ensure no clogging in the filter.
The mixed sample was inoculated with a mixture of 50% chicken processing wastewater and 50% of BOD dilution water. This mixture could help the accumulation of the biofilms.