Induction of type 1 diabetes
After seven days of adaptation, 9-week-old animals received a
single intravenous administration of STZ 45 mg/kg dissolved in
0.1 M cold citrate buffer, pH 4.5. The controls rats only received
citrate buffer. After 72 h of induction, glycemia was measured in
blood samples collected from the tail vein and the values were determined
using a glucometer. DM was defined in this study as fasting
blood glucose P200 mg/dL and animals that failed to meet this
criteriawere excluded. The fasting blood glucose (3 h) of each animal
was measured during 8 weeks protocol for monitoring diabetes.