The effectiveness of the combination of anion-exchange and
cellulose affinity chromatographic techniques for the isolation of
BG from CPHs was established for the first time. By using the
present system, the BG present at 6.6% in CPHs was purified to
84.7%. The methylation and fragmentation analyses showed that
the BG isolated from the CPHs has (1,4)/(1,3) linkage and cellotriosyl/cellotetraosyl
segment ratios of 2.60 and 2.5, respectively. The
chemical structure of the BG was also confirmed by 13C NMR spectroscopic
analysis.