STAGs.
In order to determine the percentage of each fatty acid in position
2 of the STAG the following procedurewas employed: 0.815ml
of STAGs, 375mg lipaseNovozym435 and 3.8 mlof absolute ethanol
were agitated at 300rpm in a batch reactor (Section 2.3 of this
work) for 2 h at 35 ◦C; then this reaction was then stopped by separating
the enzyme of the reaction mixture by filtration, washing
the filter with n-hexane and fitting the final volume of the product
reaction-hexane solution to 25 ml [25]. This solution was stored at
−24 ◦C until analysis by TLC and GC. 2-MAGs fromalcoholysiswere
separated by TLC and analyzed by GC. This fatty acid composition
indicated the fatty acid composition at position 2 of STAGs. The
fatty acid composition at positions 1 and 3 was determined from
this analysis and from the composition of STAGs.