Water samples for steroid analysis were collected in 1 L con¬tainers and immediately placed on ice water, transported to the laboratory and stored at -20 c. Additional water samples (lOmL) were taken (Fig. 1), placed on ice water and transported to the
laboratory for Total Ammonia-Nitrogen (TA-N), Nitrite-N, Nitrate- N and Orthophosphate-P analysis using a SAN autoanalyzer (Skalar, The Netherlands). Temperature, pH, and conductivity were mea¬sured at the sampling sites using portable meters or provided by the facilities managers. Nitrile gloves were used during all water sampling and processing activities to prevent contamination with steroids
Water samples for steroid analysis were collected in 1 L con¬tainers and immediately placed on ice water, transported to the laboratory and stored at -20 c. Additional water samples (lOmL) were taken (Fig. 1), placed on ice water and transported to the laboratory for Total Ammonia-Nitrogen (TA-N), Nitrite-N, Nitrate- N and Orthophosphate-P analysis using a SAN autoanalyzer (Skalar, The Netherlands). Temperature, pH, and conductivity were mea¬sured at the sampling sites using portable meters or provided by the facilities managers. Nitrile gloves were used during all water sampling and processing activities to prevent contamination with steroids
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