Characteristics of the MFC in the case of repeated batch
fermentation Repeated batch fermentation was carried out
without cleaning the anode surface of the MFC. After the second
batch fermentation, the formation of a biofilm on the anode surface
was confirmed. The ieV relationship for the MFC setup before and
after batch fermentation was measured for the second (Fig. 4) and
third (Fig. 5) batch fermentations. In the second batch
fermentation, the OCV, maximum current density and maximum
power density detected after fermentation (0.500 V, 19.1 mA/cm2
and 2.47 mW/cm2 (at 10.3 mA/cm2)) were also larger than those
detected before fermentation (0.460 V, 12.7 mA/cm2 and 1.56 mW/
cm2 (at 5.21 mA/cm2)), similar to the case of the first batch
fermentation (Fig. 4a). A smaller overpotential after fermentation
than that before fermentation was confirmed by the polarization
curve (Fig. 4b). The largest OCV in this study was observed before
the third batch fermentation (0.521 V). The polarization before
the third fermentation was larger than that after the second
batch fermentation; however, the maximum current and power
densities (14.4 mA/cm2 and 2.37 mW/cm2 (at 6.98 mA/cm2)) were
smaller than those detected after the second batch fermentation
(Fig. 5a). The OCV, maximum current density and maximum
power density after the third batch fermentation decreased to
0.441 V, 13.9 mA/cm2 and 1.87 mW/cm2 (at 6.65 mA/cm2),
respectively. However, the overpotential was slightly smaller than
that before fermentation (Fig. 5b).
Characteristics of the MFC in the case of repeated batchfermentation Repeated batch fermentation was carried outwithout cleaning the anode surface of the MFC. After the secondbatch fermentation, the formation of a biofilm on the anode surfacewas confirmed. The ieV relationship for the MFC setup before andafter batch fermentation was measured for the second (Fig. 4) andthird (Fig. 5) batch fermentations. In the second batchfermentation, the OCV, maximum current density and maximumpower density detected after fermentation (0.500 V, 19.1 mA/cm2and 2.47 mW/cm2 (at 10.3 mA/cm2)) were also larger than thosedetected before fermentation (0.460 V, 12.7 mA/cm2 and 1.56 mW/cm2 (at 5.21 mA/cm2)), similar to the case of the first batchfermentation (Fig. 4a). A smaller overpotential after fermentationthan that before fermentation was confirmed by the polarizationcurve (Fig. 4b). The largest OCV in this study was observed beforethe third batch fermentation (0.521 V). The polarization beforethe third fermentation was larger than that after the secondbatch fermentation; however, the maximum current and powerdensities (14.4 mA/cm2 and 2.37 mW/cm2 (at 6.98 mA/cm2)) weresmaller than those detected after the second batch fermentation(Fig. 5a). The OCV, maximum current density and maximumpower density after the third batch fermentation decreased to0.441 V, 13.9 mA/cm2 and 1.87 mW/cm2 (at 6.65 mA/cm2),respectively. However, the overpotential was slightly smaller thanthat before fermentation (Fig. 5b).
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