From the results obtained it can be concluded that it is possible to expand the existing enzyme toolbox by testing new, non-commercial enzymes produced by easy and fast procedures, which can further be immobilized by simple adsorption on economic supports. These methodologies can contribute to introduce new enzymatic tools for biotechnological applications such as biodiesel production, as they do not involve any time or solvent consumption, they are easy to conduct and sufficiently economic to be used in an eventual scaling up.