We also found a 12 day delay in the peak of
WT HIV-1 replication in other gRNA- and Cas9-
transduced cells. We measured the amount of p24 in
the culture supernatants and found similar kinetics in the
percentage of p24 intercellular antigen expression and
the amount of p24 antigen in the culture supernatants in
all cultures dually transduced by HIV-1-specific gRNA
and Cas9 lentiviral vectors (Fig. 2e). These results suggest
that the anti-HIV-1 CRISPR/Cas9 lentiviral vector system
can suppress, but not control, full HIV-1 replication.