COD measurement (% of mineralization) To understand the degree of biodegradation (mineralization) of Remazol Red reduction in chemical oxygen demand of the culture before and after decolorization after 6 h of incubation with Lysinibacillus sp. RGS were measured (25).
The nutrient medium was used as blank and similar condition was used for test. Similar procedure was employed for the
mixture of dyes and dye wastewater study.
The COD was calculated as follows: