careful evaluation of the efficiency of a selective medium should be
performed to avoid overestimated results during the development
of the study, since some culture media do not effectively select
probiotic cultures, as observed by several authors (Oberg et al.,
2011; Van De Casteele et al., 2006). It is necessary that the culture
media are previously evaluated in the matrix in question, since
dairy products such as cheese, for example, are complex matrices,
and selective media are generally evaluated in model systems using
pure cultures, which may compromise the results.
Further research need to be carried out in order to evaluate the
effectiveness of selective media, like MRS with clindamycin or bile
with higher incubation temperature as 45 C for selective counting
of L. acidophilus in Prato cheese.
careful evaluation of the efficiency of a selective medium should beperformed to avoid overestimated results during the developmentof the study, since some culture media do not effectively selectprobiotic cultures, as observed by several authors (Oberg et al.,2011; Van De Casteele et al., 2006). It is necessary that the culturemedia are previously evaluated in the matrix in question, sincedairy products such as cheese, for example, are complex matrices,and selective media are generally evaluated in model systems usingpure cultures, which may compromise the results.Further research need to be carried out in order to evaluate theeffectiveness of selective media, like MRS with clindamycin or bilewith higher incubation temperature as 45 C for selective countingof L. acidophilus in Prato cheese.
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