In conclusion, the mitigation of thermal processing contaminants like HMF and acrylamide is a very important issue for the food industry. Any approach that effectively eliminates these undesired compounds with limited effect on sensory properties of the final product is still challenging, particularly for foods like coffee products. The results of the present study revealed that yeast fermentation is a promising technique for the mitigation of HMF and acrylamide in instant coffee. The method developed by the integration of the fermentation step into the regular production of instant coffee can be easily adapted by the industry. However, it should be kept in mind that themetabolic degradation product of acrylamide is unknown for S. cerevisiae and needs further investigation.