EHP 18S rRNA gene amplification and sequence analyses
We performed a PCR targeting 18S rRNA gene from the
EHP-infected shrimp collected in Vietnam. A 1146 bp DNA fragment
was obtained. By Blast analysis, it showed 96–100% identities
to the 18S rRNA gene of EHP (GenBank Nos. KF362129, KF362130,
FJ496356, KP027539) collected from Thailand and China;
93–94% identities to an intranuclear microsporidium, Enterospora
nucleophila, 87–88% identities to other microsporidia, including
Nucleospora salmonis, Nucleospora cyclopteri and Enterocytozoon
bieneusi; 82–83% identities to Enterscytospora artemiae.