Ethanol was used to disinfect the polyethylene and remove any organic matter adhering to its surface.Treatment of polyethylene: The isolated Pseudomonas spp. (P1, P2, and P3) were inoculated in 200 ml of nutrient broth in conical flasks with 0.5 g of the polyethylene strips (natural and synthetic) amended to each flask except the blank. All the treatments were incubated in an incubator shaker at 150 rpm for 8 weeks at 40°C. The experimental design for the three Pseudomonas spp. comprised of two treatments each i.e. T1 (Pseudomonas + natural polyethylene) and T2 (Pseudomonas + synthetic polyethylene) with controls i.e. C1 (natural
polyethylene) and C2 (synthetic polyethylene) and blank (Pseudomonas). A blank for every
Pseudomonas spp. was used to examine the viability of the bacteria and check for any contamination.